磷酸酶活性分析試劑盒Phosphatase Assay kit現(xiàn)貨

英文名：Phosphatase Assay kit

儲(chǔ)存：冷藏（2-8℃）

規(guī)格：1000次/盒

品牌：子科生物ZIKER

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運(yùn)輸和保存：Shipped at ambient temperature. Upon arrival, store at 4°C. The substrate is light sensitive and should be protected from direct sunlight or UV sources. 

產(chǎn)品介紹：

The Phosphatase Assay kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases,including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases (Figure 1). The phosphatases remove the phosphate group to generate p-nitrophenol, which is deprotonated under alkaline conditions to produce pnitrophenolate that has strong absorption at 405nm. The kits components are sufficient for performing up to 1000 assays in 96-well plate format and easily adaptable to cuvettes or 384-well plates. 

磷酸酶活性分析試劑盒Phosphatase Assay kit現(xiàn)貨

Equilibrate the substrate and assay buffer to room temperature before use.

1. Make appropriate serial dilutions of your test samples with PA Assay Buffer. Prepare enough solution to perform

assays in triplicate.

2. Transfer 50μl diluted test samples to wells in a 96-well plate. In addition, use 50μl PA Assay Buffer without

enzyme for blank wells.

3. Start the reaction with the addition of 50μl PA Substrate to each well.

4. Incubate for 10-30 minutes at room temperature.

NOTE: For dynamic assay, measure the absorbance every minute at 405nm.

5. At the end of the incubation, add 50μl Stop Solution to each well to stop the reaction.

6. Measure the absorbance at 405nm. 

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磷酸酶活性分析試劑盒Phosphatase Assay kit現(xiàn)貨

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